学帮网英语翻译凝胶电泳是分析复杂蛋白质混合物、分析某一蛋白质或者多肽相对含量的一种常用方法,是一种检测蛋白质纯度、评估蛋白质分子量的强有力的生化分析方法.蛋白质电泳后聚丙烯酰
来源：学生作业学帮网 编辑：学帮网 时间：2024/05/06 06:42:10
英语翻译
凝胶电泳是分析复杂蛋白质混合物、分析某一蛋白质或者多肽相对含量的一种常用方法,是一种检测蛋白质纯度、评估蛋白质分子量的强有力的生化分析方法.蛋白质电泳后聚丙烯酰胺凝胶的染色是非常重要的步骤,考马斯亮蓝染色可以说是目前使用最为广泛的一种方法.考马斯亮蓝染色具有以下优点：一是实验成本低且不需配备昂贵的扫描设备；二是实验方案很多,可以根据自己的需要在操作简便与高灵敏度之间进行选择；三是与质谱的兼容性好.因此,此次主要试验几种适用于DNA结合蛋白电泳的考马斯亮蓝染色方法的比较.
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Gel electrophoresis is a popular method to analyze protein mixture, measure the relative amount of protein or peptide species. It is also a powerful biochemical analysis tool to detect protein purity and its molecular weight. PAGE gel staining after electrophoresis is a critical step. Coomassie stain is one of the most popular methods. It has the following advantages: First of all, low cost for the reagents and also does not require expensive scanning equipment. Secondly, many protocols can be chosen from to fit the experimental requirement to balance between simplicity and sensitivity. Last but not the least, it is also well compatible with the mass spectrometry. The current experiment compared several Coomassie stain protocols that used in DNA binding protein electrophoresis assay.